Antimicrobial properties of extracted pigments were also evaluated. Extracted pigments were analysed by TLC and various confirmation tests were carried out. Isolated pigment producing species were confirmed by fully automated Vitek 2 and Phoenix 100 system. Glycerol content was added for enhancement for good visibility of colour. Leifsonia aquatica, Pseudomonas aeruginosa, Serratia marcescens were isolated for yellow, green and red colour pigment respectively, from natural environmental source and highest colour production was achieved at 25 º C, 150 rpm in 72 h incubation time. Microbial pigments have some advantages over plant and animal species and so it seems much of importance for pigment production.

Natural colourants are generally obtained from plant, animal or microorganisms. Hence, there is an increasing demand for ' biocolour ' from natural sources which can replace artificial synthetic colours. Repeated and prolonged use of synthetic colours has thrown up many issues related to environment and human health. Finally, it is the first report of siderophore-producing microorganisms from polluted soil of Callejón de Huaylas highlands, interestedly they displayed metabolic properties useful to enhance phytoremediation and biotechnology application. Medicago sativa seedlings inoculated with BEP17-Dm, BEP18-Dm, or BRU16-Sr showed more surviving percentage under in vitro culture in presence of Cd, Pb (0.5–1.0 mM), or Al (2.5–5.0 mM). BEP18-Dm produced higher concentration of IAA (53.42 μgml⁻¹) while six bacteria and all fungi strains produced a low concentration of auxins.

subtilis BRU16-Sr exhibited higher solubilization index. Two bacteria and six fungi solubilized cadmium carbonate, while eleven bacteria and two fungi solubilized tricalcium phosphate, where P. All bacteria strains tolerated lead (2−8 mM) and eigth tolerated cadmium (1−6 mM) also all fungi tolerated lead (9−70 mM) and cadmium (3−10 mM). According to the 16S rDNA analysis, bacteria belong to Pseudomonas, Bacillus, and Achromobacter genera, whereas by ITS analysis fungi belong to Talaromyces, Hypoxylon, Tolypocladium, and Penicillium. It was selected 14 bacteria and 9 fungi strains by their iron and/or aluminum siderophore production trait, where BEP17-Dm showed higher production. Endophytic and rhizospheric microorganisms associated with six native plants adapted to heavy metal polluted soil from Punta Olímpica and Chahuapampa, located in Callejón de Huaylas mountains, were evaluated as potential candidates for technologies to clean polluted ecosystems.

Serial dilution lab report microbiology